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1. Name the detector used in a UV-VIS spectrophotometer.

2. On what basis is the wavelength selected for preparing a calibration curve?

3. What substance is used to make the cuvettes, and why?

Answer :

Final answer:

A photocoupled device is the detector used in a UV-VIS spectrophotometer. Wavelengths for calibration curves are selected based on the specific light absorbance or emission of the substance, and cuvettes are typically made of quartz because of its wide transparency range.

Explanation:

The detector used in a UV-VIS spectrophotometer can be a charge-coupled device (CCD). Wavelength selection for making a calibration curve relies on the specific wavelengths that the substance in question absorbs or emits - this can be determined through initial range finding experiments. Cuvettes used in a spectrophotometer are typically made using quartz due to its capacity to remain transparent over a wide range of wavelengths, including the ultra-violet and visible light spectra.

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