Answer :
The agarose gel is a common tool used in molecular biology to separate DNA fragments based on their size. Reovirus is a type of virus that can cause respiratory and gastrointestinal infections.
The samples will be loaded onto a 1.5% agarose gel in the following order:
1. Molecular weight ladder: The molecular weight ladder is a set of DNA fragments of known sizes that is used as a reference to determine the size of the DNA fragments in the other samples. It is loaded first so that the sizes of the other samples can be compared to it.
2. SARS-CoV: SARS-CoV, which stands for Severe Acute Respiratory Syndrome Coronavirus, is a type of virus that causes respiratory illness. It is loaded onto the gel to analyze its genetic material and compare it to the other samples.
3. Reovirus: Reovirus is a type of virus that can cause respiratory and gastrointestinal infections. It is loaded onto the gel to analyze its genetic material and compare it to the other samples.
4. Influenza virus: Influenza viruses are responsible for seasonal flu outbreaks. They are loaded onto the gel to analyze their genetic material and compare them to the other samples.
5. Rhinovirus: Rhinoviruses are a common cause of the common cold. They are loaded onto the gel to analyze their genetic material and compare them to the other samples.
By loading these samples onto the agarose gel, scientists can separate and visualize the DNA fragments present in each sample. This allows them to compare the genetic material of different viruses and determine their sizes. The agarose gel is a common tool used in molecular biology to separate DNA fragments based on their size. It is a gel made from agarose, a natural polysaccharide derived from seaweed. The gel is prepared by dissolving agarose powder in a buffer solution, which is then poured into a mold and left to solidify. Once the gel is ready, it is placed in an electrophoresis apparatus and submerged in a buffer solution. When an electric current is applied, DNA fragments in the loaded samples migrate through the gel according to their size. Smaller fragments move faster and travel further, while larger fragments move slower and stay closer to the point of loading. The DNA fragments can then be visualized using a dye that binds to the DNA and emits fluorescence under UV light. By comparing the migration of the DNA fragments in the samples to the molecular weight ladder, scientists can estimate the size of the DNA fragments in each sample. This information is crucial for understanding the genetic makeup of the viruses being studied.
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