High School

At what degrees Celsius is the steam sterilization biological indicator incubated?

A. 43-48
B. 49-54
C. 55-60
D. 61-66

Answer :

Final answer:

The steam sterilization biological indicator is typically incubated at 55-60 degrees Celsius to stimulate the growth of the indicator organism after exposure to sterilization.

Explanation:

The steam sterilization biological indicator is typically incubated at 55-60 degrees Celsius. This temperature range is chosen because it's high enough for effective sterilization without damaging the materials being processed. It's important to note that steam sterilization, commonly performed using an autoclave, works by exposing objects to high-pressure steam at 121-132 degrees Celsius. The incubation temperature for the biological indicator, however, is on a different scale and is designed to provide a suitable environment for the growth of the indicator organism after exposure to the sterilization process. Such an organism is usually a type of bacteria known to be particularly resistant to sterilization efforts, and the failure of these organisms to grow following incubation provides confidence that the sterilization process was successful.

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