Answer :
Final answer:
Pooling and running the E2 through E5 fractions of rGFP on a new Ni⁺²—agarose column could potentially increase the purity and activity of the protein but may decrease the overall yield due to potential losses in the process.
Explanation:
If the E2 through E5 fractions of the recombinant green fluorescent protein (rGFP) are combined and then applied to a new Ni⁺²—agarose column, it is likely that the purity, yield, and activity of the protein would be affected. The purity and activity of the protein could potentially increase due to the second separation process. The second Ni⁺²—agarose column would allow for further separation and filtering of the rGFP, likely leading to a more purified protein product.
However, yield may decrease due to potential losses during the second separation, as some protein could be left behind in the column or lost during the pooling and application process. However, these potential changes would be dependent upon the precise conditions and techniques of the second column separation.
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