Answer :
Final answer:
Pooling the E2 through E5 fractions and applying them onto a new Ni⁺²—agarose column can affect the purity, yield, and activity of rGFP.
Explanation:
If you pooled the E2 through E5 fractions and applied them onto a new Ni⁺²—agarose column, it would affect the purity, yield, and activity of rGFP.
Purity refers to how much of the desired protein is present without impurities. Yield refers to the amount of rGFP that is obtained.
Activity refers to the functional properties and biological activity of rGFP. When fractions are pooled and applied onto a new column, there is a possibility of contaminating impurities being carried over, which can reduce purity.
Additionally, the yield may be affected because some of the rGFP might not bind as effectively to the new Ni⁺²—agarose column.
The activity of rGFP could also be impacted if there are any changes or denaturation of the protein during the process.
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