High School

For the Disinfectant Disc Diffusion Assay, how were the plates set up?

A. The bacterial sample was swabbed onto a Mueller-Hinton agar plate.
B. The bacterial sample was swabbed onto a TSA plate.
C. The bacterial sample was swabbed onto a Mueller-Hinton agar plate.
D. The bacterial sample was added to liquid Mueller-Hinton agar, then poured.
E. The bacterial sample was added to liquid TSA, then poured.

Answer :

Final answer:

In a Disinfectant Disk Diffusion Assay, the bacterial sample is traditionally swabbed onto a Mueller-Hinton agar plate, not a TSA plate. The plate is then used to measure the effectiveness of the disinfectant.

Explanation:

In a Disinfectant Disk Diffusion Assay, the bacterial sample is usually swabbed onto a Mueller-Hinton Agar plate. Usually, a sterile cotton swab is dipped into a bacterial sample and then evenly distributed across the entire surface of the Mueller-Hinton agar plate. After swabbing, the disinfectant-infused disks are placed on the agar plate. The plate is then incubated to allow bacterial growth. The effectiveness of the disinfectant is observed as a clear zone or inhibition around the disk where the bacterial growth has been prevented by the disinfectant. This method is commonly used in clinical laboratories to test the susceptibility of microorganisms to antibiotics. Mueller-Hinton Agar is preferred over TSA (Tryptic Soy Agar) because it provides a consistent medium for susceptibility testing.

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