How will you select and grow a resistant strain of E. coli in this experiment?

A. Expose a sample of E. coli to streptomycin by inoculating it onto a streptomycin-positive plate. Any colonies that grow will carry a mutation for resistance.

B. Expose a sample of E. coli to streptomycin by inoculating it onto a streptomycin-negative plate. Any colonies that grow will carry a mutation for resistance.

C. A culture of E. coli will be mixed with streptomycin so that the antibiotic can alter the genetic composition of the bacteria.

D. Samples of bacteria are taken from the culture and observed under a microscope for signs of susceptibility or resistance. Those that are resistant are separated and plated.

The correct method to select and grow a resistant strain of E. coli in this experiment is expose a sample of E. coli to streptomycin by inoculating it onto a streptomycin positive plate. Therefore option 1 is correct.

Any colonies that grow will carry a mutation for resistance. In this method, the E. coli bacteria are exposed to streptomycin on a plate with the antibiotic.

Any colonies that grow on this plate have developed resistance to streptomycin, indicating the presence of a mutation that confers antibiotic resistance.

This approach specifically selects for resistant strains, making it a targeted and effective way to isolate and grow these strains for further study or experimentation.

Therefore option 1 is correct.

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