1. Smear preparation of cells from a solid media differs from preparation of cells from a liquid media in that the cells from a solid media are ___.

A. dispersed in a loopful of distilled water onto the microscopic slide
B. heat-killed before placing them onto the microscopic slide
C. placed directly in the center of the microscopic slide
D. stained before placing them onto the microscopic slide
E. fixed before placing them onto the microscopic slide

2. You prepared a smear of bacterial cells and stained it following the procedure, but when observing under the microscope, you did not see any cells on the slide. What could be the reason?

A. The smear was not fixed onto the slide
B. The smear was stained using a basic dye
C. The smear was too thick
D. Too much water was used to disperse the cells
E. The cells were not dispersed in distilled water before placing them onto the slide

Cells from solid media, unlike those from liquid media, need to be dispersed in distilled water before being placed on a microscopic slide. A reason for not seeing cells after staining is that the cells might have not been dispersed in distilled water before being smeared on the slide.

Explanation:

The smear preparation of cells from solid media differs from that of liquid media in that cells from solid media are A. dispersed in a loopful of distilled water onto the microscopic slide. This extra step is required for cells from solid media to help disperse the cells evenly across the slide for optimal viewing under a microscope.

As for not seeing any cells on the slide despite a proper staining procedure, there could be several factors. While a couple of factors could result, the most likely reason is E. Before placing the cells on the plate, they were not dispersed in distilled water. Dispersing cells in distilled water before the smear process helps distribute the cells evenly and thus contribute to a successful viewing under the microscope.

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