Answer :
The process described in the question involves two main steps: transforming E. coli with a cloning vector and then plating the transformed bacteria onto antibiotic media. This process is known as "Antibiotic selection." Correct Answer: D) Antibiotic selection
Explanation of the process:
1- Transforming E. coli with a cloning vector: In genetic engineering, a cloning vector is a DNA molecule, often derived from a plasmid, that can carry foreign DNA and be replicated within a host cell. E. coli, a commonly used bacterium in molecular biology, is often transformed with a cloning vector to introduce foreign DNA (such as a gene of interest) into the bacterial cells.
2- Plating onto antibiotic media: After transforming E. coli with the cloning vector, the next step is to plate the transformed bacteria onto growth media that contains an antibiotic. The cloning vector used in the transformation process typically carries a gene for antibiotic resistance, allowing the transformed E. coli to survive and grow in the presence of the antibiotic.
The process of plating transformed bacteria onto antibiotic media is called "Antibiotic selection" because only the transformed cells (those that have taken up the cloning vector) will survive and form colonies on the antibiotic-containing plates. Untransformed E. coli cells or those that did not take up the cloning vector will be unable to grow and will be killed by the antibiotic.
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Complete Question
Transforming E. coli with a cloning vector and then plating onto antibiotic media would be considered what?
A) Genetic engineering
B) Polymerase chain reaction (PCR)
C) Southern blotting
D) Antibiotic selection
