Answer :
Bacteriophages are incubated with E. coli cells before pouring onto the nutrient agar plate to allow the viruses to infect and replicate within the bacterial cells. This ensures the presence of enough phages to form visible plaques on the agar plate.
Bacteriophages are incubated with E. coli cells before pouring onto the nutrient agar plate because bacteriophages are viruses that specifically infect bacteria. By allowing the bacteriophages to incubate with E. coli cells, it gives the phages the opportunity to infect and replicate within the bacterial cells before being plated on the agar plate.
This process ensures that there will be enough phages to form visible plaques on the agar plate, which are clear areas where the host bacterial cells have been lysed.
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Because different bacteria thrive at various temperatures, incubation can be carried out at the most favourable conditions. They can enter the lag phase with the aid of nutrients and the appropriate temperature.
Incubators
A device called an incubator is used to cultivate and preserve cell or microbiological cultures. The incubator keeps the interior atmosphere's CO2 and oxygen concentrations at optimal levels together with the ideal temperature and humidity.
One should keep bacterial cultures in the incubator for at least 24 hours in order to see bacterial growth without a microscope.
Bacteriophages
Bacteriophages, also called phages, are viruses that only attack and reproduce in bacterial cells.
They are the most prevalent biological agent on earth and are widely recognised for their ubiquity in the environment.
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