Answer :
Final answer:
Proper autoclaving usually involves maintaining a temperature of 121 °C (approximately 250 degrees F) at a pressure of 15 to 20 psi, for a minimum of 20 minutes. The timing could be longer for larger volumes to ensure proper heat transfer for sterilization. Therefore, the correct answer to the question is option B. 250 degrees F for 30 minutes.
Explanation:
The question is asking for the correct temperature setting and timing for proper autoclaving, which is a method used to sterilize medical and laboratory instruments. Standard operating temperatures for autoclaves are usually 121 °C (approximately 250 degrees F) or sometimes 132 °C, but never as high as 300 degrees F or as low as 175 degrees F. These temperatures are usually maintained at a pressure of 15 to 20 psi.
The duration of autoclaving depends on the volume and type of material being sterilized, but it typically takes at least 20 minutes. Greater volumes require longer times to ensure enough heat is transferred to the material being sterilized. Therefore, the correct answer would be option b. 250 degrees F for 30 minutes.
It is important to understand that the steam in the autoclave needs to directly come into contact with the liquids or materials being sterilized for proper autoclaving. Hence, containers are left loosely closed, and instruments are loosely wrapped in paper or foil.
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