High School

Please explain how to calculate the concentration of cells in your experiment.

You spread-plated 100 µL of an overnight culture of E. coli onto LA agar plates. The next day, you counted 242 colonies on the plate.

Answer :

Final answer:

The concentration of cells in your E. coli culture is approximately 2420 CFU/mL.

Explanation:

To calculate the concentration of cells in your E. coli culture, you need to follow these steps:

  1. Spread plate a known volume of the culture onto an agar plate. In this case, you spread plated 100 uL of the overnight culture.
  2. Incubate the agar plate overnight to allow the colonies to grow.
  3. Count the number of colonies on the plate. In this case, you counted 242 colonies.
  4. Calculate the colony-forming units per milliliter (CFU/mL) using the following formula:

CFU/mL = (Number of colonies / Volume plated) x Dilution factor

In this case, the volume plated is 100 uL, and the dilution factor is 1 (assuming no dilution was performed). Therefore, the calculation would be:

CFU/mL = (242 colonies / 0.1 mL) x 1 = 2420 CFU/mL

So, the concentration of cells in your E. coli culture is approximately 2420 CFU/mL.

Learn more about calculating cell concentration here:

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