High School

The calibration curve shows a steeper slope for the theobromine samples than for the caffeine samples. Does this mean the detector is more sensitive to theobromine? Why or why not? (Consider the previous question in your answer.)

9. Based on the structures of the molecules, would you expect the UV light to interact with them differently?

10. How would the calibration curves based on peak areas be different from the ones shown? How would they be similar?

Answer :

(A).The steeper slope in the calibration curve for theobromine samples compared to caffeine samples does not necessarily mean that the detector is more sensitive to theobromine. (B). The calibration curves based on peak areas would likely show similar trends as the ones based on peak height.

The slope of the calibration curve is affected by various factors, such as the concentration of the analyte and the detector response. Thus, the difference in slope could be due to differences in the chemical properties of the two compounds that affect their behavior in the analytical system. . The peak area-based calibration curve would likely be more precise, particularly at lower concentrations, where the peaks are typically narrower and less well-defined.

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--The complete Question is, The calibration curve shows a steeper slope for the theobromine samples than for the caffeine samples. does this mean the detector is more sensitive to theobromine? why or why not? (consider the previous question in your answer.)

A. based on the structures of the molecules, would you expect the UV light to interact with them differently?

B. how would the calibration curves based on peak areas be different from the ones shown? how would they be similar? --